Batool Labibi

From

Batool Labibi

Supervisor: Liliana Attisano, collaborative internship proposed with Jeff Wrana.

The role of signaling crosstalk between TGFβ /Smad and Hippo/TAZ pathways in determining cell fate: In my research project, I plan to use a multidisciplinary approach to model the crosstalk between hippo and TGFβ/Smad signaling pathways and identify the unknown parameters of the nonlinear model using advanced control theory. The proposed research plan involves the completion of two steps: 1) identifying and modeling of crosstalk between TGFβ/Smad signaling pathways and hippo pathways 2) validation of the model in cultured cells. To achieve these goals, I will consider different scenarios for the unknown mechanisms (unmodeled dynamics in the control theory sense) and by using experimental measurements I will try to identify the unknown parameters of the models. In modeling biological networks, the unknown parameters are often fitted based on experimental measurements. However, the measurements are very noisy and limited. In this research, I will use a dynamic recursive estimator, known as Unscented Kalman Filter (UKF), to estimate the model parameters and identify the unmodeled dynamics in different scenarios. Finally, after obtaining the models to validate them, I will perform structural perturbation experiments, gene silencing and inducible overexpression. For this I will use siRNA libraries and several small molecule screens, designed and implemented in our lab to identify positive and negative chemical inhibitors of the pathways.

Personal tools
MediaWiki Appliance - Powered by TurnKey Linux